This descriptive exploratory study was conducted to describe the effect of night shift on Indian nurses at critical care units. A convenience sample of Indian nurses working at critical care units in a hospital were studied using a structured questionnaire developed by Sizeni Madide(2003) that describe the effect of night shift among nurses working in critical care units. The findings showed that female nurses had a significant difference on sufficient sleep, and interpersonal conflicts. In addition, the results indicated that nurses experience health problem and their work performance affected by the night shift. The study findings indicated that night shift affect critical care nurses well being. Consequently, this study emphasizes and urges the need to design proper nursing manager interventions that help nurses to improve nursing care quality.