Locus of control in trainee teachers is one of the important characteristic which influences teacher effectiveness and their job satisfaction. Locus of control is considered as significant factor which affects teacher's motivation. It is imperative to understand trainee teachers’ locus of control because it could account for their personal success and failure when they enter real school environment. The application of locus of control in the field of education is very significant. In case of teacher trainees, locus of control is related to motivation, performance and achievement. This study was intended to provide the idea about the nature of locus of control of male and female teacher trainees. This study was also significant because the study helps understand the personality of teacher trainees, which may help to improve the existing services or create new services. This study will significantly help encourage educators in providing teacher trainees with programmes and experiences for enhancing their internal tendencies and perceptions of both male and female teacher trainees.