( ISSN 2277 - 9809 (online) ISSN 2348 - 9359 (Print) ) New DOI : 10.32804/IRJMSH

Impact Factor* - 6.2311


**Need Help in Content editing, Data Analysis.

Research Gateway

Adv For Editing Content

   No of Download : 51    Submit Your Rating     Cite This   Download        Certificate

ESTABLISHMENT OF CALLUS CULTURES AND THEIR PHYSICO- BIOCHEMICAL ANALYSIS IN BARLERIA LUPULINA LINDL.

    1 Author(s):  SUSHIL KUMAR

Vol -  9, Issue- 11 ,         Page(s) : 244 - 252  (2018 ) DOI : https://doi.org/10.32804/IRJMSH

Abstract

Barleria lupulina Lindl. (Family-Acanthaceae) is a small shrub, commonly called Sornomukhi. It is widely distributed in South East Asia and Eastern Africa. Tribals use different parts of Barleria lupulina to treat a number of ailments and disorders. This plant is popular in Thai folk medicine because it is used externally as an anti-inflammatory agent against different viral lesions caused by varicella zoster, herpes simplex and herpes zoster viruses. It is also used to alleviate inflammation due to snake and insect bites and also has a diuretic effect and anti-amoebic properties. Nine iridoid glucosides have been isolated from different parts. Compounds like barlerin, acetylbarlerin shanzhiside methyl ester, acetylshanzhiside methyl ester, ipolamiidoside and iridoid glucosides have beenisolated and identified from the leaves of Barleria lupulina Lindl. Young leaves, petioles and stems up to 5th node from apex of Barleria lupulina grown in glass house under controlled conditions were used as explants. The explants were inoculated for establishing callus culture. For culture initiation MS medium (Murasige and Skoog, 1962) with 30 g/l sucrose and 8.0 g/l agar, supplemented with different growth regulators was used for culture establishment. Auxins viz. 2,4-D, NAA, IBA, IAA and 2,4,5-T and cytokinins viz. Kn and BAP were used individually or in combination. High callus growth response with compact green callus was recorded on MS + 1.0mg/l NAA + 0.5mg/l BAP as compared to other growth regulator combinations in Barleria lupulina. Barleria lupulina calli subcultured on 1.0mg/l NAA with 1.0 and 2.0mg/l BAP resulted in higher protein, nitrogen and total sugar content after 4 weeks, though with lesser callus growth response than on NAA+0.5 mg/l BAP.

  • Balkwill, M.J. and Balkwill, K. (1998). A Preliminary Analysis of Distribution Patterns in a Large, Pantropical Genus, Barleria L. (Acanthaceae). Journal of Biogeography 25:95-110.
  • De Feudis, F.V., Papadopoulos, V., Drieu, K. (2003). Ginkgo biloba extracts and cancer: a research area in its infancy. Fundam Clin Pharmacol 17: 405-417.
  • Doss, A., Parivuguna, V., Vijayasanthi, M. and Sruthi, S. (2011). Antibacterial evaluation and phytochemical analysis of certain medicinal plants, Western Ghats, Coimbatore. J Res Biol 1: 24-29.
  • Gill, L.S., Idu, M. and Ogbar, B.N. (1997). Folk medicinal plants practices and belief of the Bini people in Nigeria. Ethanoboany, 9: 1-5.
  • Gupta, M., Mazumder, U.K., Rath, N., Mukhopadhyay, D.K. (2000). Antitumour activity of methanolic extract of Cassia fistula L. seed against Ehrlich ascites carcinoma. J. Ethnopharmacol. 72: 151-156.
  • Issaabadi, Z., Nastrollahzadeh, S. and Sajadi, M. (2016). Green synthesis of the copper nanoparticles supported on bentonite and investigation of its catalytic activity. J cleaner production.
  • Kanchanapoom, T., Kasai, R. and Yamasaki, K. (2001). Iridoid glucosides from Barleria lupulina. Phytochemistry 58: 337-341.
  • Kaur, P.K., Vasisht, K., Karan, M. (2014). HPTLC Method for Shanzhiside esters: Simultaneous quantitative analysis of barlerin, acetylbarlerin and shanzhiside methyl ester in Barleria species. J Chromatograph Separat Techniq 5: 246.
  • Kumari R., and R.C., Dubey. (2016a). Phytochemical analysis and antibacterial and cytotoxic properties of Barleria lupulina Lindl. extracts. Journal of Plant pathology and Microbiology, 7(10): 1-6.
  • Kumari, R., Dubey, R.C. (2016). HPTLC and GC-MS profile of B. lupulina Lindl. extracts and their effect on enteric bacterial pathogens. J App Pharm 8: 61-68.
  • Kutchan, T.M. (2001). Ecological, Arsenal and Environmental Dispatcher. The paradigm of secondary metabolism. Plant Phys. 125: 58-60.
  • Lans, C., Harper, T., Georges, K. and Bridgewater, E. (2001). Medicinal and ethnoveterinary remedies of hunters Trinidad. BMC Complementary and Alternative Medicine 1: 10.
  • Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. (1951). "Protein measurement with the Folin phenol reagent". Journal of Biological Chemistry, 193 (1): 265–75. PMID 14907713.
  • Mazumder, P.M., Mondal, A., Sasmal, D., Arulmozhi, S., Rathinavelusamy, P. (2012). Evaluation of antiarthritic and immunomodulatory activity of Barleria lupulina. Asian Pac J Trop Biomed S1400-S1406.
  • Mazumder, P.M., Paramaguru, R., Mohanty, A., Sasmal, D. (2014). Evaluation of in vitro anticataract activity and aldose reductase potential of Barleria lupulina Lindl. Pharmacologia 5: 172-176.
  • Moghaddam, M.S., Kumar, P.A., Reddy, G.B., Ghole, V.S. (2005). Effect of diabecon on sugar-induced lens opacity in organ culture: mechanism of action. J Ethnopharmacol 97: 397-403.
  • Moin, S., Babu, S.S., and A. Mahalakshmipriya. (2012). In vitro callus production and antibacterial activity of Barleria lupulina Lindl. Asia Pacific Journal of Molecular Biology and Biotechnology, 20(2): 59-64.
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63
  • Murashige, T. and Skoog, F. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum 15: 473–497.
  • Nelson, N. (1944).  A photometric adaptation of the Somogyi method for the determination of glucose.  J. Bio. Chem., 163, 375.
  • Oomah, B.D. (2003). Isolation, characterization and assessment of secondary metabolites from plant for use in Human Health PBI Bulletin 1: 1-4.
  • Pattanayak, S., Pal, S., Mandal, T.P., Debnath, P.K., Bandyopadhyay S.K. (2014). A comparative study of extract of succulent leaves of living plant with methanolic and aqueous extract of Barleria lupulina Lindl. Against pathogenic microbes by disc diffusion and Spectrophotometry. Exploratory Animal and Medical Research, 4(2): 148-157.
  • Sawangjaroen, N., Phongpaichit, S., Subhadhirasakul, S., Visutthi, M., Srisuwan, N. and Thammapalerd, N. (2006). The anti-amoebic activity of some medicinal plants used by AIDS patients in southern Thailand. Parasitology Research 98: 588-592.
  • Smitinand, T. (2001). Thai Plant Names, Revised Edition, the Forest Herbarium, Royal Forest Department, Bangkok.
  • Snell, F.E. and Snell, C.T. (1949). Colorimetric Methods of Analysis 3rd Edn. Vol.1. pp. 785-807 Van, Nostrand, Company Inc.Toronto, New York, London.
  •  Suba, V., Munegesan, T., Pal. M., Mandal, S.C., and Saha B.P. (2004). Antiulcer activity of methanol fraction of Barleria lupuliva Lindl. in animal models. Phytother. Res. John willey & sons 18 (11): 925-929.
  • Yoosoo K,C., Panpisutochi, Y., chaichanna, S. and Santisuk T. (1999). Evalucation of anti-HSV-2 activities of Barleria lupulina and Dinacanthus nutans. J. Ethanopharmacol. Pub-Elsevier. 67 (2):  179-187.

*Contents are provided by Authors of articles. Please contact us if you having any query.

Testimony


Bank Details