Quality of Worklife is a multimodal comparative concept influenced by time, place and personal and social values. This study targets the women professionals working in Madurai and examines their Quality of worklife balance experiences. Thus maintaining Worklife Balance is necessary for the working women to have a good quality of worklife.The aim of this research is to identify the impact of Worklife Balance over the Quality of Worklife variables.The factors like Stress, Conflict, Work Environment, job Satisfaction are taken for this study.Percentage Analysis,Descriptive Statistics,Anova,Regression analysis were used for analyzing the data.The result shows that the organization should concentrate on reducing level of stress underwent by the women working in the organization. It also suggested that the organization may conduct stress reduction program for working women.